How much do Volatile Nanites do to reduce enemy damage? Against structures only? Planets too? Anything in the gravity well?
Volatile Nanites do not reduce their damage; it increases the damage they receive from your attacks. You can always reference this thread for capital ship abilities:
https://forums.sinsofasolarempire.com/335807
It increases the damage they suffer 30%.
How much does Phasic barrier reduce damage?
I'm actually not 100% sure of the value. What I do know is that it's mostly used for its annoying effect on the targeting AI rather than for damage reduction. When activated, any unit ordered to attack the structure will pick a new target, usually moving to attack something else. This requires manual control by the opponent to override and actually destroy the structures efficiently. In terms of damage reduction, it's far less than the passive bonus offered by the Advent Hanger's shield bestowal.
Enslaved Labor looks like a loser: costs 800 + 150 resources ~ 1,500 on a balanced map, so must spend 37,500 to pay off. If my income is 25/s (reasonable early to middle game average?), takes 1,500 seconds = 25 minutes
Yup, and not all costs apply to enslaved labour, either. Research, capital ship promotion, and planet upgrades are all exempt. It's not terrible, and as your income pushes higher this upgrade improves further, but in general you can't afford this early on. If your resource income is really high (such that you're selling to make credits meet) then it becomes a lot more sensible, since it helps you avoid using the black market.
Am I right that once I have wormhole jumps may my max trade route length will be about 3 since almost everything is adjacent to a wormhole?
If you're playing without the expansion pack, then yes this is the case. If you're playing with the expansion pack, this is only true if you have starbases on both sides of the wormhole with trade port module upgrades.